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Title:
A rapid and robust tri-color flow cytometry assay for monitoring malaria parasite development
Authors:
Malleret, Benoît; Claser, Carla; Ong, Alice Soh Meoy; Suwanarusk, Rossarin; Sriprawat, Kanlaya; Howland, Shanshan Wu; Russell, Bruce; Nosten, Francois; Rénia, Laurent
Affiliation:
AA(Laboratory of Malaria Immunobiology, Singapore Immunology Network (SIgN), Agency for Science, Technology and Research (A*STAR), Biopolis, Singapore), AB(Laboratory of Malaria Immunobiology, Singapore Immunology Network (SIgN), Agency for Science, Technology and Research (A*STAR), Biopolis, Singapore), AC(Laboratory of Malaria Immunobiology, Singapore Immunology Network (SIgN), Agency for Science, Technology and Research (A*STAR), Biopolis, Singapore), AD(Laboratory of Malaria Immunobiology, Singapore Immunology Network (SIgN), Agency for Science, Technology and Research (A*STAR), Biopolis, Singapore), AE(Mahidol-Oxford University Tropical Medicine Research Programme, Shoklo Malaria Research Unit, Mae Sot, Thailand), AF(Laboratory of Malaria Immunobiology, Singapore Immunology Network (SIgN), Agency for Science, Technology and Research (A*STAR), Biopolis, Singapore), AG(Laboratory of Malaria Immunobiology, Singapore Immunology Network (SIgN), Agency for Science, Technology and Research (A*STAR), Biopolis, Singapore), AH(Mahidol-Oxford University Tropical Medicine Research Programme, Shoklo Malaria Research Unit, Mae Sot, Thailand), AI(Laboratory of Malaria Immunobiology, Singapore Immunology Network (SIgN), Agency for Science, Technology and Research (A*STAR), Biopolis, Singapore)
Publication:
Nature Scientific Reports, Volume 1, id. 118 (2011).
Publication Date:
10/2011
Origin:
NATURE
Abstract Copyright:
(c) 2011: Macmillan Publishers Limited. All rights reserved
DOI:
10.1038/srep00118
Bibliographic Code:
2011NatSR...1E.118M

Abstract

Microscopic examination of Giemsa-stained thin blood smears remains the gold standard method used to quantify and stage malaria parasites. However, this technique is tedious, and requires trained microscopists. We have developed a fast and simple flow cytometry method to quantify and stage, various malaria parasites in red blood cells in whole blood or in vitro cultured Plasmodium falciparum. The parasites were stained with dihydroethidium and Hoechst 33342 or SYBR Green I and leukocytes were identified with an antibody against CD45. Depending on the DNA stains used, samples were analyzed using different models of flow cytometers. This protocol, which does not require any washing steps, allows infected red blood cells to be distinguished from leukocytes, as well as allowing non-infected reticulocytes and normocytes to be identified. It also allows assessing the proportion of parasites at different developmental stages. Lastly, we demonstrate how this technique can be applied to antimalarial drug testing.
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Database: Astronomy
Physics
arXiv e-prints